Establishing a callogenesis protocol aimed at obtaining somatic embryos in leaf explants of African mahogany
Abstract
African mahogany (Khaya grandifoliola) is a forest species of considerable economic interest, whose clonal propagation may benefit from the development of efficient tissue culture protocols. The present study aimed to evaluate callus induction in African mahogany leaves using concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and benzylaminopurine (BA). The optimal response occurred at a 2,4-D concentration of 67.86 μM, with predominantly friable characteristics, concentrated mainly in the midrib region, and to a lesser extent along the lateral margins of the explant. The BA concentration of 11.31 μM, in turn, promoted the formation of more compact calli, with white coloration, also predominating in the midrib region, with lower occurrence on the lateral margins of the explant.
KEYWORDS: Khaya grandifoliola, in vitro regeneration, plant growth regulators, plant biotechnology.
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