Compensatory growth of Piaractus mesopotamicus raised in net cages and subjected to a period of fasting and refeeding

Authors

Abstract

The present study evaluated the effects of feed restriction on production, blood and tissue parameters of Piaractus mesopotamicus raised in net cages. The experiment was divided into two phases. In phase I, which lasted for 30 days, 960 P. mesopotamicus (368.44 ± 155.05 g) were distributed in a completely randomised design in six net cages with two treatments and three replications each, namely: fish subjected to 15 days of feed restriction followed by 15 days of feeding (FR) and fish fed continuously (CF). In phase II, which lasted for 60 days, 600 P. mesopotamicus (780.40 ± 96.07 g) were distributed in six net tanks, with the same treatments as were used in phase I. Growth performance parameters did not differ significantly between treatments (p > 0.05) in both experimental phases. However, feed intake was significantly lower in the FR fish (p < 0.05). In phase I, higher haemoglobin and erythrocyte levels were observed in the FR fish compared to the CF fish (p < 0.05). In phase II, no significant differences (p > 0.05) were observed in blood parameters between treatments. The hepatocyte histomorphometric parameters of pacu demonstrated no significant variation between treatments (p > 0.05) across both phases. The protocol of biweekly cycles of feed restriction followed by refeeding did not harm the growth of P. mesopotamicus raised in net cages and had minimal effects on blood and hepatocyte histomorphometric parameters.
Keywords: feeding strategy, fish farming, neotropical fish, pacu.

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1 Acunha RMG, Simões ARP, Oliveira FC, Fernandes CEDS, Soares MP, Barros AFD, Campos CMD. Food restriction in hybrid catfish Pseudoplatystoma reticulatum x Leiarius marmoratus produced in cages: zootechnical performance, physiological metabolism and economic viability. Acta Scientiarum. Animal Sciences. 2025;47: e73187. https://doi.org/10.4025/actascianimsci.v47i1.73187

2 Mohanta KN, et al. Effect of restricted feeding and refeeding on compensatory growth, nutrient utilization and gain, production performance and whole body composition of carp cultured in earthen pond. Aquac Nutr. 2017;23(3):460–9. Available from: https://doi.org/10.1111/anu.12414

3 Ali M, Nicieza A, Wootton RJ. Compensatory growth in fishes: a response to growth depression. Fish Fish. 2003;4(2):147–90. Available from: https://doi.org/10.1046/j.1467-2979.2003.00120.x

4 McCue MD. Starvation physiology: reviewing the different strategies animals use to survive a common challenge. Comp Biochem Physiol A Mol Integr Physiol. 2010;156(1):1–18. Available from: https://doi.org/10.1016/j.cbpa.2010.01.002

5 Favero GC, Gimbo RY, Montoya LNF, Carneiro DJ, Urbinati EC. A fasting period during grow-out make juvenile pacu (Piaractus mesopotamicus) leaner but does not impair growth. Aquaculture. 2020;524:735242. Available from: https://doi.org/10.1016/j.aquaculture.2020.735242

6 Yengkokpam S, Sahu NP, Pal AK, Debnath D, Kumar S, Jain KK. Compensatory growth, feed intake and body composition of Labeo rohita fingerlings following feed deprivation. Aquac Nutr. 2014;20(2):101–8. Available from: https://doi.org/10.1111/anu.12056

7 Oliveira LCC, et al. Feeding strategy induces compensatory growth in Heros severus fingerlings, an Amazonian ornamental fish. Aquac Rep. 2020;18:100436. Available from: https://doi.org/10.1016/j.aqrep.2020.100436

8 Silva WS, et al. Effects of cyclical short-term fasting and refeeding on juvenile Lophiosilurus alexandri, a carnivorous Neotropical catfish. Aquaculture. 2019;505:12–7. Available from: https://doi.org/10.1016/j.aquaculture.2019.02.047

9 Assis YPAS, Assis PL, Melo NFAC, Palheta GDA, Luz RK, Favero GC. Feed restriction as a feeding management strategy in Colossoma macropomum juveniles under recirculating aquaculture system (RAS). Aquaculture. 2020;529:735689. Available from: https://doi.org/10.1016/j.aquaculture.2020.735689

10 Bull CD, Metcalfe NB. Regulation of hyperphagia in response to varying energy deficits in overwintering juvenile Atlantic salmon. J Fish Biol. 1997;50(3):498–510. Available from: https://doi.org/10.1111/j.1095-8649.1997.tb01945.x

11 Takahashi LS, Biller JD, Criscuolo-Urbinati E, Urbinati EC. Feeding strategy with alternate fasting and refeeding: effects on farmed pacu production. J Anim Physiol Anim Nutr. 2011;95(2):259–66. Available from: https://doi.org/10.1111/j.1439-0396.2010.01050.x

12 Sevgili H, Hoşsu B, Emre Y, Kanyilmaz M. Compensatory growth after various levels of dietary protein restriction in rainbow trout, Oncorhynchus mykiss. Aquaculture. 2012;344:126–34. Available from: https://doi.org/10.1016/j.aquaculture.2012.03.030

13 Carriquiriborde P, De Luca JC, Dulout FN, Ronco AE. Nucleolar variation in response to nutritional condition in juvenile pejerrey Odontesthes bonariensis (Valenciennes). J Fish Biol. 2007;70(3):947–58. Available from: https://doi.org/10.1111/j.1095-8649.2007.01357.x

14 Rodrigues RA, Saturnino KC, Fernandes CE. Liver histology and histomorphometry in hybrid sorubim (Pseudoplatystoma reticulatum × Pseudoplatystoma corruscans) reared on intensive fish farming. Aquac Res. 2017;48(9):5083–93. Available from: https://doi.org/10.1111/are.13325

15 Godoy MP. Peixes do Brasil: subordem Characoidei. Bacia do Rio Mogi-Guassu. Piracicaba: Franciscana; 1975. 216 p.

16 Gomes LC, Baldisserotto B. Espécies nativas para piscicultura no Brasil: Tambaqui (Colossoma macropomum). In: Baldisserotto B, editor. Espécies nativas para piscicultura no Brasil. 3rd ed. Santa Maria: UFSM; 2019. p. 147–68

17 Kojima JT, et al. Short periods of food restriction do not affect growth, survival or muscle development on pacu larvae. Aquaculture. 2015;436:137–42. Available from: https://doi.org/10.1016/j.aquaculture.2014.11.004

18 Paula TGD, et al. Food restriction increases the expression of mTORC1 complex genes in the skeletal muscle of juvenile pacu (Piaractus mesopotamicus). PLoS One. 2017;12(5):e0177679. Available from: https://doi.org/10.1371/journal.pone.0177679

19 Favero GC, Gimbo RY, Franco Montoya LN, Zanuzzo FS, Urbinati EC. Fasting and refeeding lead to more efficient growth in lean pacu (Piaractus mesopotamicus). Aquac Res. 2018;49(1):359–66. Available from: https://doi.org/10.1111/are.13466

20 Rotta MA. Aspectos gerais da fisiologia e estrutura do sistema digestivo dos peixes relacionados à piscicultura. Corumbá: Embrapa Pantanal; 2003. 49 p. (Embrapa Pantanal. Boletim de Pesquisa, 53).

21 Ferreira AL, Bonifácio CT, Silva WS, Takata R, Favero GC, Luz RK. Anesthesia with eugenol and menthol for Piaractus brachypomus (Cuvier, 1818): induction and recovery times, ventilation frequency and hematological and biochemical responses. Aquaculture. 2021;544:737076. Available from: https://doi.org/10.1016/j.aquaculture.2021.737076

22 Collier HB. Standardization of blood haemoglobin determinations. Can Med Assoc J. 1944;50(6):550–2.

23 Goldenfarb PB, Bowyer FP, Hall E, Brosious E. Reproducibility in the hematology laboratory: the microhematometric determination. Am J Clin Pathol. 1971;56:35–9. Available from: https://doi.org/10.1093/ajcp/56.1.35

24 Torfi-Mozanzadeh M, Marammazi JG, Yaghoubi M, Yavari V, Agh N, Gisbert E. Somatic and physiological responses to cyclic fasting and re-feeding periods in sobaity sea bream (Sparidentex hasta, Valenciennes 1830). Aquac Nutr. 2017;23(1):181–91. Available from: https://doi.org/10.1111/anu.12379

25 Urbinati EC, Sarmiento SJ, Takahashi LS. Short-term cycles of feed deprivation and refeeding promote full compensatory growth in the Amazon fish matrinxã (Brycon amazonicus). Aquaculture. 2014;433:430–3. Available from: https://doi.org/10.1016/j.aquaculture.2014.06.030

26 Favero GC, Boaventura TP, Ferreira AL, Silva AC, Porto LA, Luz RK. Fasting/re-feeding and water temperature promote the mobilization of body reserves in juvenile freshwater carnivorous catfish Lophiosilurus alexandri. Aquaculture. 2021;511:734223. Available from: https://doi.org/10.1016/j.aquaculture.2019.734223

27 Passinato EB, et al. Performance and analysis of the production of Nile tilapia submitted to different feeding management. Semina Ciênc Agrár. 2015;36(6 Suppl):4481–92. Available from: https://doi.org/10.5433/1679-0359.2015v36n6Supl2p4481

28 Roa FGB, et al. Production performance of tambaqui juveniles subjected to short feed-deprivation and refeeding cycles. Bol Inst Pesca. 2019;45(4):1–9. Available from: https://doi.org/10.20950/1678-2305.2019.45.4.466

29 Navarro I, Gutiérrez J. Fasting and starvation. In: Hochachka PW, Mommsen TP, editors. Biochemistry and molecular biology of fishes. Vol. 4. Amsterdam: Elsevier; 1995. p. 393–434. Available from: https://doi.org/10.1016/S1873-0140(06)80020-2

30 Lermen CL, et al. Effect of different temperature regimes on metabolic and blood parameters of silver catfish Rhamdia quelen. Aquaculture. 2004;239(1–4):497–507. Available from: https://doi.org/10.1016/j.aquaculture.2004.06.021

31 Godavarthy P, Kumari YS, Bikshapathy E. Starvation induced cholesterogenesis in hepatic and extra hepatic tissues of climbing perch, Anabas testudineus (Bloch). Saudi J Biol Sci. 2012;19(4):489–94. Available from: https://doi.org/10.1016/j.sjbs.2012.07.004

32 Kim JH, Jeong MH, Jun JC, Kim TI. Changes in hematological, biochemical and non-specific immune parameters of olive flounder, Paralichthys olivaceus, following starvation. Asian-Australas J Anim Sci. 2014;27(9):1360. Available from: https://doi.org/10.5713/ajas.2014.14110

33 Figueroa RI, Rodríguez-Sabarís R, Aldegunde M, Soengas JL. Effects of food deprivation on 24 h-changes in brain and liver carbohydrate and ketone body metabolism of rainbow trout. J Fish Biol. 2000;57(3):631–46. Available from: https://doi.org/10.1111/j.1095-8649.2000.tb00265.x

34 Chatzifotis S, Papadaki M, Despoti S, Roufidou C, Antonopoulou E. Effect of starvation and re-feeding on reproductive indices, body weight, plasma metabolites and oxidative enzymes of sea bass (Dicentrarchus labrax). Aquaculture. 2011;316(1–4):53–9. Available from: https://doi.org/10.1016/j.aquaculture.2011.02.044

35 Hevrøy EM, Azpeleta C, Shimizu M, et al. Effects of short-term starvation on ghrelin, GH-IGF system, and IGF-binding proteins in Atlantic salmon. Fish Physiol Biochem. 2011;37:217–32. Available from: https://doi.org/10.1007/s10695-010-9434-3

36 Jobling M, Johansen SJS. Lipostat, hiperfagia e crescimento de recuperação. Aquac Res. 1999;30(7):473–8. Available from: http://doi.org/10.1046/j.1365-2109.1999.00358.x

37 Won ET, Borski RJ. Regulação endócrina do crescimento compensatório em peixes. Front Endocrinol. 2013;4:74. Available from: https://doi.org/10.3389/fendo.2013.00074

38 Farias KNN, et al. Effects of days-fasting and refeeding on growth, biochemical and histometric liver parameters in pacu Piaractus mesopotamicus. Braz J Biol. 2024;84:e287072. Available from: https://doi.org/10.1590/1519-6984.287072

39 Hung SS, Liu W, Li H, Storebakken T, Cui Y. Effect of starvation on some morphological and biochemical parameters in white sturgeon, Acipenser transmontanus. Aquaculture. 1997;151(1–4):357–63. Available from: https://doi.org/10.1016/S0044-8486(96)01506-2

40 Tamadoni R, Nafisi Bahabadi M, Morshedi V, Bagheri D, Mozanzadeh MT. Effect of short-term fasting and re-feeding on growth, digestive enzyme activities and antioxidant defence in yellowfin seabream, Acanthopagrus latus (Houttuyn, 1782). Aquac Res. 2020;51(4):1437–45. Available from: https://doi.org/10.1111/are.14489

41 Burgos-Aceves MA, Lionetti L, Faggio C. Multidisciplinary haematology as prognostic device in environmental and xenobiotic stress-induced response in fish. Sci Total Environ. 2019;670:1170–83. Available from: https://doi.org/10.1016/j.scitotenv.2019.03.275

42 Nikinmaa M, Cech JJ, McEnroe M. Blood oxygen transport in stressed striped bass (Morone saxatilis): role of beta-adrenergic responses. J Comp Physiol B. 1984;154:365–9. Available from: https://doi.org/10.1007/BF00684443

43 Prisingkorn W, et al. Transcriptomics, metabolomics and histology indicate that high-carbohydrate diet negatively affects the liver health of blunt snout bream (Megalobrama amblycephala). BMC Genomics. 2017;18:856. Available from: https://doi.org/10.1186/s12864-017-4246-9

44 Liu Y, et al. Resveratrol inclusion alleviated high-dietary-carbohydrate-induced glycogen deposition and immune response of largemouth bass, Micropterus salmoides. Br J Nutr. 2022;127(2):165–76. Available from: https://doi.org/10.1017/S0007114521000544

45 Ostaszewska T, et al. Growth and morphological changes in the digestive tract of rainbow trout (Oncorhynchus mykiss) and pacu (Piaractus mesopotamicus) due to casein replacement with soybean proteins. Aquaculture. 2005;245(1–4):273–86. https://doi.org/10.1016/j.aquaculture.2004.12.005

46 Gisbert E, Ortiz-Delgado JB, Sarasquete C. Nutritional cellular biomarkers in early life stages of fish. Histol Histopathol. 2008;23(12):1525–39. https://doi.org/10.14670/hh-23.1525

47 Darias MJ, Gómez MA, Tello S, Gisbert E. Growth, survival and the histology of the digestive tract of juvenile Osteoglossum bicirrhosum (Cuvier, 1829) fed three diets containing different protein and lipid levels. J Appl Ichthyol. 2015;31(4):67–73. Available from: https://doi.org/10.1111/jai.12977

Published

2026-06-16

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ANIMAL SCIENCE

How to Cite

DAVALO, M. R. S. et al. Compensatory growth of Piaractus mesopotamicus raised in net cages and subjected to a period of fasting and refeeding. Brazilian Animal Science/ Ciência Animal Brasileira, v. 27, 16 Jun.2026.

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