MORPHOLOGICAL EVALUATION OF RABBIT CORNEA CRYOPRESERVED WITH ETHYLENE GLYCOL

Authors

  • Lorena Borges Alves Universidade Federal de Uberlândia (UFU), Uberlândia, Minas Gerais, Brasil
  • Duvaldo Eurides Universidade Federal de Uberlândia (UFU), Uberlândia, Minas Gerais, Brasil
  • Lilian Vieira de Andrade Universidade Federal de Uberlândia (UFU), Uberlândia, Minas Gerais, Brasil
  • Luiz Antônio F. da Silva Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brasil
  • Benito J. N. Alves de Oliveira Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brasil
  • Luiz Augusto de Souza Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brasil

DOI:

https://doi.org/10.5216/cab.v12i2.11445

Keywords:

Animal sanity

Abstract

Rabbit corneas, cryopreserved with ethylene glycol, were evaluated. Corneas of the right and left ocular globes were collected from eleven New Zelanda male rabbits, weighing between 4.0kg and 5.0kg, immediately after the animals were slaughtered. The corneas were removed with a 2.0mm edge of sclera and washed in physiological solution 0.9%. The right corneas were fixed in formaldeide, enclosed in paraffin, stained in hematoxyline eosine for evaluation in light microscopy. The left corneas were immersed in cryoprotect bottles containing phosphate-buffered saline solution, 0.4% of bovine albumen and 1.5 molar of ethylene glycol. The bottles were frozen for 60 days at -196º C in liquid nitrogen. After thawing,  they were submitted to macrocospic analysis, and measured from digital images. The rabbits´ corneas preservated with ethylene glycol in liquid nitrogen caused no structural nor morphometry alterations at the layers that compose the cornea.

KEYWORDS: córnea; cryopreserved; rabbit.

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Author Biographies

  • Lorena Borges Alves, Universidade Federal de Uberlândia (UFU), Uberlândia, Minas Gerais, Brasil

     

  • Benito J. N. Alves de Oliveira, Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brasil

     

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Published

2011-06-25

Issue

Vol. 12 No. 2 (2011)

Section

Veterinary Medicine

License

Copyright (c) 2011 Brazilian Animal Science/ Ciência Animal Brasileira

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How to Cite

ALVES, L. B. et al. MORPHOLOGICAL EVALUATION OF RABBIT CORNEA CRYOPRESERVED WITH ETHYLENE GLYCOL. Brazilian Animal Science/ Ciência Animal Brasileira, v. 12, n. 2, p. 376–381, 25 Jun.2011.