EFECT OF MINIMUM CONTAMINATION MEDIUM (MMC) AND MODIFIED EGG YOLK LACTOSE (LGM) EXTENDERS ON CANINE SEMEN VIABILITY AFTER COOLING AT 4 ºC FOR 24 HOURS IN EQUITAINER®

Authors

  • Bianca Perez Barcellos Universidade Federal Fluminense (UFF), Niterói, Rio de Janeiro, Brasil
  • Marco Antônio Rocha Universidade Federal Fluminense (UFF), Niterói, Rio de Janeiro, Brasil
  • Luciano Jamas Polícia Militar do Rio de Janeiro
  • Felipe Zandonadi Brandão Universidade Federal Fluminense (UFF), Niterói, Rio de Janeiro, Brasil
  • Luis Garcia Nogueira Universidade Federal Fluminense (UFF), Niterói, Rio de Janeiro, Brasil
  • Tania Goes de Pinho Universidade Federal Fluminense (UFF), Niterói, Rio de Janeiro, Brasil

DOI:

https://doi.org/10.5216/cab.v12i1.4692

Keywords:

Animal reproduction

Abstract

The aim of this study was to compare the viability of canine semen after dilution in Minimum Contamination Medium (MMC) and Modified Lactose-Egg yolk extender (LGM) and incubation at 4 ºC for 12 and 24 hours. Thirteen ejaculates were collected from 5 adult dogs by digital manipulation. Macroscopic and microscopic characteristics were assessed right after collection. Semen was divided to be diluted (1:3) in MMC and LGM and subsequently chilled in Equitainer®. Seminal parameters of motility and spermatic morphology, membrane integrity (hyposmotic test), spermatic viability and spontaneous acrosome reaction (Trypan-blue Giemsa stain) were evaluated in fresh and chilled semen after 12 and 24 hours of incubation at 4 ºC.  No difference between extenders was identified in semen conservation after 12 hours. After 24 hours just spermatic motility was different (p>0.05). But, after both periods of conservation, semen diluted in LGM maintained most of the characteristics verified in the fresh semen. The mean of true and false acrosome reaction did not exceed 2% in both semen extenders, which demonstrates no influence of media component and incubation period on these phenomena at 4 ºC. In conclusion, these results indicate that both extenders can be used in canine semen conservation at 4 ºC in contêiner during 12 hours without significant changes in semen characteristics.

KEYWORDS: Dogs, sperm, cooling.

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Published

2011-03-31

Issue

Vol. 12 No. 1 (2011)

Section

Veterinary Medicine

License

Copyright (c) 2011 Brazilian Animal Science/ Ciência Animal Brasileira

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How to Cite

BARCELLOS, B. P. et al. EFECT OF MINIMUM CONTAMINATION MEDIUM (MMC) AND MODIFIED EGG YOLK LACTOSE (LGM) EXTENDERS ON CANINE SEMEN VIABILITY AFTER COOLING AT 4 ºC FOR 24 HOURS IN EQUITAINER®. Brazilian Animal Science/ Ciência Animal Brasileira, v. 12, n. 1, p. 126–133, 31 Mar.2011.