PICROSIRIUS RED AND MASSON'S TRICHROME STAINING TECHNIQUES AS TOOLS FOR DETECTION OF COLLAGEN FIBERS IN THE SKIN OF DOGS WITH ENDOCRINE DERMATOPATHOLOGIES

Glícia Meneses Costa; Steffi Lima Araujo; Francisco Antônio Félix Xavier Júnior; Glayciane Bezerra de Morais; João Alison de Moraes Silveira; Daniel de Araújo Viana; Janaina Serra Azul Monteiro Evangelista

doi:10.1590/1089-6891v20e-55398

PICROSIRIUS RED AND MASSON'S TRICHROME STAINING TECHNIQUES AS TOOLS FOR DETECTION OF COLLAGEN FIBERS IN THE SKIN OF DOGS WITH ENDOCRINE DERMATOPATHOLOGIES

Authors

Glícia Meneses Costa Universidade Estadual do Ceará (UECE), Fortaleza, Ceará, Brasil https://orcid.org/0000-0001-6498-5337 (unauthenticated)
Steffi Lima Araujo Universidade Estadual do Ceará (UECE), Fortaleza, Ceará, Brasil https://orcid.org/0000-0001-7953-0570 (unauthenticated)
Francisco Antônio Félix Xavier Júnior Universidade Estadual do Ceará (UECE), Fortaleza, Ceará, Brasil https://orcid.org/0000-0002-2635-1306 (unauthenticated)
Glayciane Bezerra de Morais Universidade Estadual do Ceará (UECE), Fortaleza, Ceará, Brasil https://orcid.org/0000-0002-3627-7939 (unauthenticated)
João Alison de Moraes Silveira Universidade Federal do Ceará (UFC), Sobral, Ceará, Brasil https://orcid.org/0000-0002-4502-1214 (unauthenticated)
Daniel de Araújo Viana Laboratório PATHOVET - Anatomia Patológica e Patologia Clínica Veterinária LTDA, Fortaleza, Ceará, Brasil https://orcid.org/0000-0002-0505-5700 (unauthenticated)
Janaina Serra Azul Monteiro Evangelista Universidade Estadual do Ceará (UECE), Fortaleza, Ceará, Brasil https://orcid.org/0000-0002-9583-1573 (unauthenticated)

DOI:

https://doi.org/10.1590/1089-6891v20e-55398

Abstract

Canine endocrinopathies, such as hypothyroidism and hyperadrenocortism,induce typical dermatological alterations. Collagen fibers are significant for the maintenance of structural integrity,as well as in the determination of tissue function. This study aimed at assessing the coloration caused by Picrosirius Red staining under circular polarization and Masson Trichrome staining, as tools to quantify the total collagen in the skin of dogs exhibiting endocrine dermatopathies. Skin samples taken from dogs with hypothyroidism and hyperadrenocorticism were stained using Hematoxylin and Eosin (HE), Masson's Trichrome (MT) and Picrosirius Red (PSR). The animals with hyperadrenocorticism revealed a higher percentage of collagen area than did the animals with hypothyroidism PSRp (hypothyroidism = 43.76 ± 0.8255 and hyperadrenocorticism = 47.08 ± 0.8584). The percentage of the collagen area using MT between the groups is given in parenthesis (hypothyroidism = 52.06 ± 0.9371, hyperadrenocorticism = 61 ± 0.7529 and control = 56.88 ± 0.64) (p <0.05). It is therefore, evident that the special stains employed are useful in estimating the percentage of collagen area in the skin, as they revealed that dogs with hypothyroidism had lower collagen deposition whereas those animals with hyperadrenocorticism showed higher quantity of collagen in the dermis.
Keywords: canine endocrinopathies, skin, fibrosis,Picrosirius Red, polarization

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Author Biographies

Glícia Meneses Costa, Universidade Estadual do Ceará (UECE), Fortaleza, Ceará, Brasil
Steffi Lima Araujo, Universidade Estadual do Ceará (UECE), Fortaleza, Ceará, Brasil

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Published

2019-09-23

Issue

Vol. 20 (2019): Continuous publication

Section

VETERINARY MEDICINE

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How to Cite

COSTA, G. M. et al. PICROSIRIUS RED AND MASSON’S TRICHROME STAINING TECHNIQUES AS TOOLS FOR DETECTION OF COLLAGEN FIBERS IN THE SKIN OF DOGS WITH ENDOCRINE DERMATOPATHOLOGIES. Brazilian Animal Science/ Ciência Animal Brasileira, v. 20, p. 1–10, 23 Sep.2019.